The fresh new SH QTL to the chromosome 3 was also similar to the QTLs to have En ce qui concerne (Additional document step 1: Desk S2)

The fresh new SH QTL to the chromosome 3 was also similar to the QTLs to have En ce qui concerne (Additional document step 1: Desk S2)

Another location towards chromosome 7 to have SH try sensed from the evaluation the people from the screenhouse

On inhabitants Kalarata/NSIC Rc238, a total of five high QTLs towards chromosomes step 3, 5, six, eight, and you may 8 had been identified to own En ce qui concerne around each other testing conditions (Dining table dos). Around three high QTLs was in fact identified to possess SH for the chromosomes 1, 3, and you can 7, from which QTLs toward chromosomes 1 and you can step 3 were thought of during the both tests criteria.

The new Sur QTLs qSUR5–1 (named as qSUR5–step one

The tolerant parent Kalarata contributed the SUR QTLs qSUR3–1Rc238-SCR-fourteen, qSUR6–1Rc238-SCR-fourteen, and qSUR7–1Rc238-SCR-14 while the QTLs qSUR5–1Rc238-SCR-14 and qSUR8–1 Rc238-SCR-14 came from susceptible parent NSIC Rc238 under screen house condition at 14 DAS (Table 2). Same five QTLs showed effect on survival at 14 DAS under tray-on-table condition and at 21 DAS in both screening conditions. The largest effect QTL qSUR7–1 (named as qSUR7–1Rc238-SCR-fourteen, qSUR7–1Rc238-TAB-14, qSUR7–1Rc238-SCR-21 and qSUR7–1Rc238-TAB-21) showed a LOD score of to and explained % to % of the phenotypic variation, with an additive effect of % to %, followed by qSUR6–1 (named as qSUR6–1Rc238-SCR-fourteen, qSUR6–1Rc238-TAB-fourteen, qSUR6–1Rc238-SCR-21 and qSUR6–1Rc238-SCR-21), which explained % to % of the phenotypic variation, detected by a LOD score of 5.97 to 6.96, and had an additive effect of 9.79% to%. qSUR3–1 (named as qSUR3–1Rc238-SCR-fourteen, qSUR3–1Rc238-TAB-fourteen, qSUR3–1Rc238-SCR-21 and qSUR3–1Rc238-TAB-21) contributed % to % of the phenotypic variation, with an additive effect of % to %. Rc238-SCR-fourteen, qSUR5–1Rc238-TAB-fourteen, qSUR5–1Rc238-SCR-21 and qSUR5–1Rc238-TAB-21) and qSUR8–1 (named as qSUR8–1Rc238-SCR-fourteen, qSUR8–1Rc238-TAB-fourteen, qSUR8–1Rc238-SCR-21 and qSUR8–1Rc238-TAB-21) from high-yielding susceptible parent NSIC Rc238 contributed 9.76% to % and % to % of the phenotypic variation, with LOD scores of 4.10 to 4.57 and 4.78 to 5.21, respectively (Table 2, Additional file 1: Figure S2).

All SH QTLs were contributed by Kalarata, the parent with anaerobic germination potential. The largest effect QTL identified for SH is qSH1–1 (named as qSH1–1Rc238-SCR-21 and qSH1–1Rc238-TAB-21), which is consistent over both screening conditions. It explained a phenotypic variation of 12.5% to 22.1%, with a LOD score of 5.4 to 9.4. The second largest effect QTL detected for SH was consistent with SUR QTL qSH3–1 (named as qSH3–1Rc238-SCR-14, qSH3–1Rc238-SCR-21 and qSH3–1Rc238-TAB-21,), which was also consistent in both screening conditions. This QTL explained phenotypic variation of 10.1% to14.4%, with a LOD score of 3.7 to 5.8 and an additive effect of 0.84% to 5.17%. QTL qSH7–1Rc238-SCR-21 was identified with a LOD score of 4.6 and explained 10.7% of the phenotypic variation and had an additive effect of 4.57%. It was identified only under screenhouse conditions and was located in a different position from the identified QTL (qSUR7–1) for SUR (Additional file 1: Table S2, Additional file 1: Figure S3A). Under controlled conditions, no QTL was detected for germination, whereas one QTL on chromosome 1 (qSH1-2Rc238-CON-21) was detected for SH with an LOD score of 6.1 that could explain 14.1% of the phenotypic variation (Additional file 1: Table S2). Altogether, the identified QTLs for SUR explained phenotypic variation in the range of % to % while that for the trait SH ranged from 10.1% to 36.5% for the Kalarata/NSIC Rc238 population.

Toward populace Kalarata/NSIC Rc222, all in all, around three tall QTLs into the chromosomes step three, six, and 7 was indeed thought of to have En ce qui concerne around both tests conditions, which have been also similar to the earliest people (Table step three). A couple significant QTLs have been detected having SH towards chromosomes step 1 and you may step three, at which new QTL for the chromosome 1, seen below one another tests standards, was also normal with the first populace for the same characteristic. In all instances, parent Kalarata with anaerobic germination prospective provided the brand new tolerant alleles having AG (Even more document step 1: Dining table S3).